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Identifying the microbiome composition from primary tissues directly affords an opportunity to study the causative relationships between the host microbiome and disease. However, this is challenging due the low abundance of microbial DNA relative to the host. We present a systematic evaluation of microbiome profiling directly from endoscopic biopsies by whole genome sequencing. We compared our methods with other approaches on datasets with previously identified microbial composition. We applied this approach to identify the microbiome from 27 stomach biopsies, and validated the presence of Helicobacter pylori by quantitative PCR. Finally, we profiled the microbial composition in The Cancer Genome Atlas gastric adenocarcinoma cohort.
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To address any question about the human microbiome, the microbiota needs to be sampled, and temporal and spatial scales need to be considered before undertaking this ,Microbial diversity of healthy humans. The diversity of microbes within a given body habitat can be defined as the number and abundance distribution of distinct types ,To assess factors influencing the success of whole-genome sequencing for mainstream clinical diagnosis, we sequenced 217 individuals from 156 independent cases or ,Microbiomes of the dust particles collected from the International Space Station and Spacecraft Assembly Facilities,Welcome to the Data Analysis and Coordination Center (DACC) for the National Institutes of Health (NIH) Common Fund supported Human Microbiome Project (HMP).,The human body harbours a varied, complex, and dynamic community of microorganisms known as the human microbiome. The term microbiome was originally coined ,Abstract. High-throughput sequencing technology is rapidly improving in quality, speed and cost. It is therefore becoming more widely used to study whole communities ,16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles,Highlights Human microbiome has emerged as a major player in regulating health and disease. Accurate identification of microbes constituting the microbiome is ,Identifying the microbiome composition from primary tissues directly affords an opportunity to study the causative relationships between the host microbiome and disease.
Identification of low abundance microbiome in clinical
Identifying the microbiome composition from primary tissues directly affords an opportunity to study the causative relationships between the host microbiome and disease.
Analysis of the microbiome: Advantages of whole genome
Highlights Human microbiome has emerged as a major player in regulating health and disease. Accurate identification of microbes constituting the microbiome is
16S rRNA gene pyrosequencing of reference and clinical
16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles
High throughput sequencing methods and analysis for
Abstract. High-throughput sequencing technology is rapidly improving in quality, speed and cost. It is therefore becoming more widely used to study whole communities
WAO Journal | Full text | The microbiome of the human
The human body harbours a varied, complex, and dynamic community of microorganisms known as the human microbiome. The term microbiome was originally coined
Human Microbiome Project DACC - Tools & Protocols
Welcome to the Data Analysis and Coordination Center (DACC) for the National Institutes of Health (NIH) Common Fund supported Human Microbiome Project (HMP).
Microbiomes of the dust particles collected from the
Microbiomes of the dust particles collected from the International Space Station and Spacecraft Assembly Facilities
Factors influencing success of clinical genome sequencing
To assess factors influencing the success of whole-genome sequencing for mainstream clinical diagnosis, we sequenced 217 individuals from 156 independent cases or
Structure, Function and Diversity of the Healthy Human
Microbial diversity of healthy humans. The diversity of microbes within a given body habitat can be defined as the number and abundance distribution of distinct types
The Human Microbiome Project : Article : Nature
To address any question about the human microbiome, the microbiota needs to be sampled, and temporal and spatial scales need to be considered before undertaking this
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